Code: BIOL-154
Semester: B Semester
Course Type: Core
Course website: Course Website
ECTS units: 6
Hours per week: Theory - 4 hours
Instructor
Professor: Tzamarias Dimitrios
Email: t
Office phone number: +30 2810 394057
Lab phone number: +30 2810 394050
Description
Course contents: Fundamental Biochemical mechanisms acting in common in divergent species. Eukaryotic vs procaryotic cell, cell architecture, model organisms. Common molecular design yet great phenotypic differences. DNA is the basis of biological diversity of life. Chemical composition of cells. Bio-molecules and chemical bonds. Weak interactions, hydrogen bonds, water, solubility. pH regulation in biological solutions, Henderson-Hasselbalc equation. Structure and function of proteins. Structure of amino acids, the basis of the 3D protein structure. Primary, secondary, tertiary structure. Peptide bond, a-helix, b-sheet, loops etc, Protein folding, quaternary structure. Methods for studying protein structure and function. Methods for Protein purification, and detection, immune-methods, NMR, X-ray analysis. Biochemical evolution. Homology of 3-D protein structures, evolution trees, ancient DNA. Enzymes. Basic concepts of bio-catalysis and reaction kinetics. Free energy, transition state, active center, co-enzymes. Michaelis-Menten model, modes of enzymatic inhibition. Regulatory strategies Proteolytic enzymes, protein kinases, restriction endonuclases. DNA, RNA and genetic information. Nucleic acids, double-helix structure, phosphor-diester bond. DNA replication, gene structure, gene expression, translation. Methods for studying gene expression. Restriction enzyme, electrophoresis, DNA sequencing, PCR, DNA recombination technics. Basic Metabolism. Connected reactions and regulation. Oxidation of food, energy production. ATP: structure and energy content. Glycolysis. Biochemical reactions and regulation. Fermentation: Mechanism and physiological role. Pentose phosphate pathway. Oxidative and no oxidative stage, physiological role, associated genetic disease. Krebs cycle. Chemical reactions and regulation. Oxidative phosphorylation. Basic concepts and regulation. |