Associate Professor
PHD: 

2003, University of Crete

Scientific interest: 
Biochemistry, Molecular Immunology, Molecular Biology, Epigenetics, Chromatin structure and function, cell nuclear organization
Phones
Office phone number: 
+30 2810 391163
Lab phone number: 
+30 2810 391173
Fax: 
+30 2810 391101
Office: 
Α 251 (IMBB-FORTH)
Lab: 
Α 204 (IMBB-FORTH)

Courses

Full CV

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Education

1998-2003:           
PhD in Molecular Biology and Biomedicine, Department of Biology - University of Crete, Greece. Laboratory of Prof. Joseph Papamatheakis.
 
1996-1998:           
MSc in Molecular Biology, Department of Biology - University of Crete, Greece. Laboratory of Prof. Joseph Papamatheakis.
 
1992-1996:           
Bachelor in Biology, Department of Biology - University of Crete, Greece.

Career

Current Positions
11/2016-present: Associate Professor of Molecular Biology & Epigenetics
09/2009-10/2016: Assistant Professor of Biochemistry, Department of Biology - University of Crete, Greece (Tenured 03/2013)
09/2009-present: Principal Investigator, Institute of Molecular Biology and Biotechnology (IMBB-FORTH), Greece
 
Previous Positions
2008-2009: Research Assistant Professor, Institute of Molecular Biology and Biotechnology (IMBB-FORTH), Greece
2003-2007: Postdoctoral Fellow, Section of Immunobiology, Yale University School of Medicine and   Howard Hughes Medical Institute, New Haven, USA. Laboratory of Prof. Richard A. Flavell.

Scientific interests

Regulation of gene expression can be accomplished “in cis ” by several regulatory elements exerting their action on physically linked genes on the same chromosome. A “ trans” regulation aspect of gene expression has also recently been brought to light by studies of the interactions and function of regulatory elements located on a chromosome different from the one that carries the regulated gene. Such regulatory elements include enhancers or locus control regions that are shown to be able to regulate the expression not only of cis linked genes but also of genes located on different chromosomes, in trans.

The main objective of our laboratory is to apply a combination of biocomputing, molecular, biochemical, imaging and genetic approaches in order to identify and characterize protein complexes that generate and/or maintain long range chromosomal interactions in cell populations of the innate and adaptive immune system. We aim to provide substantial information on how the genome is shaped as a whole and how the nuclear structure patterns in the context of distinct subnuclear microenvironments regulate global gene expression (3D epigenetics).

Recent publications

HiChIP and Hi-C Protocol Optimized for Primary Murine T Cells.
Zelenka T, Spilianakis C. 
Methods Protoc. 2021; 4(3):49. doi: https://doi.org/10.3390/mps4030049 

SATB1-mediated chromatin landscape in T cells. 
Zelenka T & Spilianakis C.G. 
Nucleus 2020; 11: 117-131; doi: https://doi.org/10.1080/19491034.2020.1775037

Developmental Conservation of microRNA Gene Localization at the Nuclear Periphery.  
Salataj E, Stathopoulou  C, Hafþórsson  RA, Nikolaou  C, Spilianakis  C.G.
PLoS One 2019; 14(11):e0223759.    https://doi.org/10.1371/journal. pone.0223759 

The BACH1-HMOX1 Regulatory Axis Is Indispensable for Proper Macrophage Subtype Specification and Skeletal Muscle Regeneration.  
Patsalos A, Tzerpos P, Halasz L, Nagy G, Pap A, Giannakis N, Lyroni K, Koliaraki V, Pintye E, Dezso B, Kollias G, Spilianakis CG, Nagy L.
J Immunol. 2019; 203(6):1532-1547 . doi: https://doi.org/10.4049/jimmunol.1900553

Long non-coding RNA SeT and miR-155 regulate the Tnfα gene allelic expression profile. 
Stathopoulou C, Kapsetaki M, Stratigi K, Spilianakis C.
PLoS One 2017; 14;12(9):e0184788. doi: https://doi.org/10.1371/journal.pone.0184788 

Coordinated Regulation of miR-155 and miR-146a Genes during Induction of Endotoxin Tolerance in Macrophages.  
Doxaki C., Kampranis S.C., Eliopoulos A.G., Spilianakis C., Tsatsanis C. 
J Immunol. 2015; 195(12):5750-5761. doi: 10.4049/jimmunol.1500615

Spatial proximity of homologous alleles and long noncoding RNAs regulate a switch in allelic gene expression.  
Stratigi K., Kapsetaki M., Aivaliotis M., Town T., Flavell RA., Spilianakis CG.
Proc Natl Acad Sci USA 2015; 112(13):E1577-1586 doi: https://doi.org/10.1073/pnas.1502182112