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Methods for the functional analysis of biological macromolecules
Code:
BIOL-251
Semester:
D Semester
Course type:
Obligatory
Course units:
2
ECTS units:
3
Hours per week
Laboratory:
3
Instructors
Description
Objectives of the course: The primary objective of this course is for students to (1) learn fundamental approaches for experimentally investigating biological macromolecules, (2) learn the theoretical foundations for the methods used, and (3) understand the applicability of the biochemical methods to realistic situations.
Course contents
- DNA ligation and cloning (3 hours)
- Transformation of bacteria (3 hours)
- Lysis of bacterial cells expressing alkaline phosphatase (3 hours)
- Enzyme purification with ion-exchange chromatography (4 hours)
- Enzyme identification using polyacrylamide gel electrophoresis (3 hours)
- Enzyme action (3 hours)
- Polymerase Chain Reaction (3 hours)
- Genotyping (3 hours)
- DNA Hybridization using Southern Ι (3 hours)
- DNA Hybridization using Southern Ι (3 hours)
- Immunological methodologies (4 hours)
Undergraduate Announcements
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05/11/2018 - 12:13
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15/10/2018 - 15:31
13/02/2017 - 12:41
Undergraduate courses
Biology department
The Department of Biology of the University of Crete was established in 1981 and is today an internationally recognized center for contemporary university education and research in the field of Biology.
University of Crete, Voutes University Campus, GR-70013,P.O.Box 2208, Heraklion, Crete, Greece
Tel.: +30 2810 394400-3, Fax: +30 2810 394404,
E-mail: secbio@uoc.gr
Tel.: +30 2810 394400-3, Fax: +30 2810 394404,
E-mail: secbio@uoc.gr