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Methods in Microbiology and Biochemistry

Code: 
BIOL-209
Semester: 
C Semester
Course type: 
Obligatory
Course units: 
2
ECTS units: 
3

Hours per week

Instructors

Associate Professor

Description

Course contents: Aseptic conditions in microbiology – preparation and sterilization of growth media. Microscopes – Microscopic observation of microorganisms – Stains. Quantitative measurement of bacterial cells using serial dilutions - Bacterial growth in liquid cultures –  Growth curves. Antibiotics/Resistance mechanisms. Influence of exogenous factors on bacterial growth. Chemical components of blood - Titration of blood and plasma. Use of spectrophotometers – Protein quantitation – Enzyme kinetics. Electrophoresis: Preparation of polyacrylamide and agarose gels. Electrophoresis: Staining and molecular weight determination. Thin Layer Chromatography (amino acids and lipids).

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Biology department

The Department of Biology of the University of Crete was established in 1981 and is today an internationally recognized center for contemporary university education and research in the field of Biology.

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