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General methods for the identification and analysis of biological macromolecules

Code: 
BIOL-155
Semester: 
B Semester
Course type: 
Obligatory
Special notes: 
Υπεύθυνος Εργαστηριακού μαθήματος: Δ.Τζαμαρίας
Course units: 
2
ECTS units: 
3

Hours per week

Laboratory: 
4

Instructors

Associate Professor

Description

Objectives of the course: The primary objective of this course is for students to (1) learn fundamental approaches for experimentally investigating biological macromolecules, (2) learn the theoretical foundations for the methods used, and (3) understand the applicability of the biochemical methods to realistic situations

Course contents

  1. Preparation of solutions (3 hours)
  2. Acid-Base Titrations and Neutralization reactions (3 hours)
  3. Redox (reduction-oxidation) reactions-Spectroscopic methods (3 hours)
  4. Synthesis of Aspirin (4 hours)
  5. Quantification of Protein concentration (4 hours)
  6. The detection of Fats, Proteins and Carbohydrates in Foods (4 hours)
  7. Digestion (fats, proteins, carbohydrates), general methods for characterizing the products (4 hours)
  8. Extraction, chromatographic identification and absorption spectra of photosynthetic pigments (3 hours)
  9.  Extraction of plasmid DNA (3 hours)
  10. Quantification, electrophoresis and digestion of plasmid DNA (3 hours)
  11. Extraction of eukaryotic RNA (3 hours)

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The Department of Biology of the University of Crete was established in 1981 and is today an internationally recognized center for contemporary university education and research in the field of Biology.

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