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Applications of Current Microscopy Techniques

Code: 
BIOL-493
Semester: 
Spring Semester
Course type: 
Selection
Course units: 
2
ECTS units: 
4

Hours per week

Theory: 
2

Instructors

Associate Professor

Description

Objectives of the course (preferably expressed in terms of learning outcomes and competences):  To understand the optical principles involved in light microscopy, the basic components of modern microscopes and imaging systems and their applications in answering questions of cell biology.

Course contents: Fundamentals of light microscopy; lenses and optics; resolution and image formation; dark-field, polarisation, phase contrast and Differential Interference Contrast microscopy; fluorescence microscopy; confocal laser scanning, 2-photon and video microscopy; Fluorescence resonance energy transfer, Fluorescence lifetime imaging, Fluorescence recovery after photobleaching, Photoactivation, Total internal reflection fluorescence.

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The Department of Biology of the University of Crete was established in 1981 and is today an internationally recognized center for contemporary university education and research in the field of Biology.

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