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Advanced methods for the analysis of cellular processes

Code: 
BIOL-300
Semester: 
E Semester
Course type: 
Obligatory
Course units: 
2
ECTS units: 
3

Hours per week

Theory: 
3

Instructors

Professor
Associate Professor

Description

Objectives of the course: The primary objective of this course is for students to (1) learn fundamental approaches for experimentally investigating cellular processes, (2) learn the theoretical foundations for the methods used, and (3) understand the applicability of the biochemical methods to realistic situations.

Course contents

  1. Function of cell membranes-ΑΤΡase activity (3 hours)
  2. Measuring the water potential of plant cells (3 hours)
  3. Molecular Genetics assays in yeast I (3 hours)
  4. Molecular Genetics assays in yeast II (3 hours)
  5. Isolation of lymphocytes from mouse spleen (3 hours)
  6. Lymphoid and myeloid cell morphology (3 hours)
  7. Immunization of experimental mice (4 hours)
  8. Blood groups (3 hours)
  9. Phagocytosis/lymphocyte response in mitogens (3.5 hours)
  10. Cytotoxicity (4 hours)
  11. Observing cell division with fluorescence microscopy (3 hours)

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Biology department

The Department of Biology of the University of Crete was established in 1981 and is today an internationally recognized center for contemporary university education and research in the field of Biology.

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