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Cell Biology

B Semester
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Hours per week





Objectives of the course (preferably expressed in terms of learning outcomes and competences): Cell biology of animal cells

Course contentsEukaryotic Cells: evolution. Biological Membranes: lipid bilayer, fluidity and asymmetry of the bilayer, membrane proteins, membrane transport, carrier proteins, ion channels. Mitochondrion: membranes, functional specialization of internal compartments, oxidation, chemiosmotic process, the respiratory chain and ATP-synthase, the genome of mitochondria. Endoplasmic Reticulum (ER): Rough ER, Smooth ER, direction of signal peptides to the Rough ER, topology of multipass transmembrane proteins, soluble proteins in the ER, N-linked glycosylation in ER. Golgi Apparatus: ER - Golgi - communication, O-linked glycosylation in Golgi, oligosaccharide chains processing in Golgi, secretory vesicles, synaptic vesicles. Lysosomes: Transport from trans Golgi to lysosomes, transport of lysosomal enzymes. Peroxisomes: Oxidative reactions, import of proteins into peroxisomes. Endocytosis / Vesicular Transport: Endosomes, pinocytic vesicles, Clathrin-coated pits, receptor mediated endocytosis, coatomer-coated vesicles, GTP-binding proteins in vesicular transport. Cell Nucleus: Membranes of the nuclear envelope, nuclear pore, transport of macromolecules, chromosomal DNA and its packaging, the global structure of chromosomes, nucleolus. Cytoskeleton: The nature and function of cytoskeleton, intermediate filaments, microtubules (microtubule-associated proteins, motor proteins and movements, centrioles and basal bodies), actin filaments (actin binding proteins, motor proteins, microvilli, migration of animal cells, muscle contraction). Extracellular Space: Cell junctions, cell-cell adhesion, the extracellular matrix. Cell-Division Cycle: The general strategy  and phases of the cell cycle, the cell-cycle control system, cell-division controls in multicellular animals, growth factors, mitosis, cytokinesis. MeiosisLaboratory Practicals: Light and Electron Microscopy.

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The Department of Biology of the University of Crete was established in 1981 and is today an internationally recognized center for contemporary university education and research in the field of Biology.

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